sequence of blood draw tubes
Blood Draw Order Tube ColorDescription ShowHide All. If several specimens are to be taken at one time the following sequence of blood collection should be recommended.
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Blood Draw Order Tube Color NameAdditive of Inversions.
. Draw Royal Blue top tubes in the order indicated by the additive. Blood culture tube or bottle. Sodium citrate tubes eg blue-stopper Serum tubes with or without clot activator with or without gel separator eg red- gold- speckled-stopper Heparin tubes with or without gel eg green-stopper EDTA tubes eg lavender-stopper Glycolytic inhibitor tubes eg gray-stopper.
SST Serum Separator Tube 4 5. Serum As requested by laboratories such as for Hams Test Cold Agglutinins Donath-Landsteiner Cryoglobulins. Order of draw chart tubes must be drawn in order of sequence 1 through 10 red black as required red section i 1 tube each light blue section ii 1 tube 1 tube each for all.
363095 KFK168 Draw Volume 27ml Cat. Trace Elements Trace Elements 368381 Draw Volume 6ml Cat. Color Varies Blood Cultures.
Draw Royal Blue top tubes in the order indicated by the additive. When collecting more than one tube type always collect in the following order. Blood Cultures Invert 8 to 10 times 2.
The following list lists the. Gently mix all tubes as they are collected by inverting each tube 5-8 times. Collect Aerobic then Anaerobic.
Blue top Tube contains sodium Citrate. The order of draw is important because the act of filling itself can sometimes affect the blood sample through. They are listed by chemical additives that have been introduced to the tube by the manufacturer to perform a specific function when mixed with the blood.
For needle and syringe order of draw refer to Prompt Document. In phlebotomy this term has special meaning as it is the order in which certain tubes can be used. 367837 KFK168 Draw Volume.
Collect the blood into the containers in the order listed below for evacuated systems. Fluoride Oxalate Glucose Alcohol Lactate 368920 Draw Volume 2ml Mix 3-4 Times Mix 8-10 Times Mix 5-6 Times Mix 6 Times Mix 8-10. The reason why tubes are color-coded is for practical and.
EDTA Blood Transfusion Group Screen Crossmatch Antenatal blood group DATDCT KleihauerFetal Cell Count 367941 Draw Volume 6ml Cat. There are seven tubes which make up the order of draw. When collecting diagnostic blood specimens by venipuncture into evacuated tubes the order of draw is b ased on Clinical and Laboratory Standards Institutes CLSI standard H3-A6 from November 2007 as follows.
1 Blood cultures yellow SPS sterile 2 Light blue buffered sodium citrate tube 3. The blue bottle is used for haematology tests involving the clotting system which require inactivated whole blood for analysis. The majority of blood collection tubes contain an ingredient that either speeds up the bloods clotting clot activator or stops blood from becoming clot-free anticoagulant.
Fill to frosted fill line on tube. A blood collection tube is a sterile glass or plastic test tube with a coloured rubber or plastic top. The correct order of draw follows.
Lavender Tube contains K2EDTA additive used for hematology testing. Order of draw refers to the sequence with which one fills the blood tubes either through the needle holder or through the syringe. Plasma gel sodium potassium bicarbonate chloride albumin all thyroid analysis total protein creatinine urea calcium billirubin alt gamma gt alkaline phosphatase ld amylase creatine.
Sterileblood cultures yellow stopper or bottles most institutions use bottles Considering the most common tubes used by nurses for venipuncture here is the proper order of blood draw. Blood Collection order of draw for tubes and bottles Tube Type Tests Instructions Blood Culture Bottles Blood Culture Ensure aseptic technique. Monterey CA 93940 Blood Tube Selection and Order of Draw Blood Tube Selection and Order of Draw 1.
Gray top tube 6 mL Na Flouride 9. Order of Draw When Using Evacuated Tubes. Light Blue Sodium Citrate.
Sodium citrate tube eg blue closure Serum tubes including those with clot activator and gels eg red red-speckled gold closures Heparin tube with or without gel eg. Soy trypsin or soy trypsin inhibitor. Blood collection set for venipuncture and a coagulation citrate tube is the first specimen tube to be drawn a discard tube should be drawn first.
Blood Cultures 8 to 10 times Serum glass tube Citrate 3 to 4 times BD SST Gel Separator Tube 5 times BD SST Gel Separator Tube Serum plastic tube Heparin 8 to 10 times BD PST Gel Separator Tube With Heparin EDTA 8 to 10 times Fluoride glucose tube 8 to 10 times BD Vacutainer Systems Preanalytical Solutions 1 Becton Drive. Red Clot Activator. The flow of blood needs to be steady but not forced when filling tubes.
Light Green Lithium Heparin. This top creates a vacuity inside the tube allowing a predefined volume of a blood sample to be drawn. Yellow top tube 85 mL ACD solution A 10.
Inadvertent contamination of the sample through handling- In other words if you are taking a blood culture if you stick your needle in a. For Cold Agglutinins Donath-Landsteiner sample must be tansported at 37C. Tubes with ACD are for cellular studies HLA typing paternity testing.
CANNOT be collected via capillary collection method. Tubes with additives blood is collected from different additive tubes in the following order. Tube MUST contain venous blood only.
Dark Blue top tube 2 mL Fibrin Degradation Product FDP tube. Vacutainer tubes may have anticoagulating reagents that help in stabilising and preserving the sample prior to processing. Contact Blood Transfusion 20339 in advance.
Binds and remove calcium to prevent blood from clotting. Sodium Fluoride Potassium Oxalate. Evacuated Tubes that are used for blood collection for different laboratory tests are made up of tubes in variable sizes and color-coded tops to indicate tube contents.
It follows the principle which is most commonly known as the order of draw Different tests and biochemical assays require varying types of sample collection tubes. Laboratory tube collection is a process applied when withdrawing blood samples from patients before they get tested in the laboratory. Dark Green Sodium Heparin.
7 mL No additive - Red strip on label - Draw as 4. Draw Volume 4ml Cat. 3 mL no additive must be used ONLY if drawing a citrate blue tube with a tube.
Capillary tubes Used for whole blood tests andor point of care testing blood gasses. 7 mL No additive - Red strip on label - Draw as 4 6 mL K2-EDTA - Blue strip on label - Draw as 7.
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